STRAIN INFORMATION


Family Identifier
NMF267
Synonym(s)
Roranmf267; Rorasg-3J; C57BL/6J-nmf267/J; JR#5047
Synopsis
The mutants are small, with splayed hind limbs, and unable to walk continuously upright.
Gene Symbol
Rora (View MGI Record)
Gene
RAR-related orphan receptor alpha
Allele Symbol
Rora (View MGI Record)
Allele
neuroscience mutagenesis facility267
Abnormal Assay(s)
Assay: Overt movement/posture

   Domain(s): Visible Mutants ; Movement/Neuromuscular Function
Phenotype Description
The mutants are small and have splayed hind limbs, which can be observed at approximately 3.6 weeks of age (mean 3.7+/-2.2 weeks; n=18). When moving around they have a tendency to lean and eventually fall over to the side, at times sliding for some distance, before they regain a walking position. These mutants are fragile, and a colony needs to be maintained through ovarian transplants. Complementation analysis with B6.Cg-Rorasg/J (JR#2651) has shown NMF267 to be an allele of Rora (RAR-related orphan receptor alpha), i.e. a heterozygote x heterozygote mating resulted in 2 mutants in a total of 9 progeny.
Pathology Report
Standard pathology work-up on five mutants (22- 46 days of age) revealed a disorganized Purkinje cell layer and a loss of granule cells.
HERITABILITY AND MAPPING INFORMATION
Background Strain
C57BL/6J
Heritability Mode
Recessive
Heritability Status
Proven; 3 heterozygote x heterozygote matings produced 8 affected mice in a total of 27 progeny.
Chromosome
9
Molecular Interval
see Rora
Map
STATUS INFORMATION
Mutant Status
Cryopreserved Embryos
Comments
(45) NMF267 have been requested by and distributed to 4 investigators.
Contact e-Mail Address
nmf-mice@jax.org