STRAIN INFORMATION
Family Identifier
 NMF244
Synonym(s)
 Myo5anmf244; A.B6 Tyr+-nmf244/J; A.B6 Tyr+-Myo5ad-l31J/J; JR#5012
Synopsis
 The mutants show ‘hot-foot’ like behavior and a dilute brown coat.
Gene Symbol
 Myo5a (View MGI Record)
Gene
 Myosin Va
Allele Symbol
 Myo5anmf244 (View MGI Record)
Allele
 Neuroscience mutagenesis facility244
Abnormal Assay(s)
 Assay: Coat/Fur

   Domain(s): Visible Mutants

Assay: Overt movement/posture

   Domain(s): Movement/Neuromuscular Function
Phenotype Description
 The mutants are small with impaired hind limb movement, i.e. 'hot-foot' like behavior (of one or both limbs), and show agouti dilute coat color. These mice are fragile and usually die by or before 5 weeks of age; a colony needs to be maintained through ovarian transplants. Complementation tests between heterozygous NMF244 and DLS/LeJ mice produced six affected mice in a total of 22 progeny, suggesting that NMF244 represents a new allele of dilute-lethal in the Myo5a gene. These affected mice show a more severe phenotype than NMF244, i.e. they intermittently lean to the side, fall over, but are able to regain their walking position; they also show a non-agouti grey coat color. The coat color appears to result from a non-agouti grey allele that is known to exist in heterozygotes for the dilute-lethal allele of Myo5a, and becomes apparent in the presence of the agouti dilute allele, carried by the female NMF244.
Pathology Report
 Standard pathology work-up on three mutants (23 - 29 days of age) revealed no abnormalities.
HERITABILITY AND MAPPING INFORMATION
Background Strain
 A.B6-Tyr+
Heritability Mode
 Recessive
Heritability Status
 Proven; 2 heterozygote x heterozygote matings produced 7 agouti dilute mice in a total of 27 progeny; 6 of the 7 agouti dilute mice showed hot-foot like behavior.
Chromosome
 9
Molecular Interval
 see Myo5a
Map
STATUS INFORMATION
Mutant Status
 Cryopreserved Embryos
Comments
 Please note: The majority of frozen NMF embryos is obtained through matings between homozygous (or heterozygous) mutant males and wild-type females; if recovery of mutants is requested, the mice will be shipped as soon as possible following wean without further phenotype or genotype testing. NMF embryos are supplied subject to the General Terms and Conditions of Sale posted at www.jaxmice.jax.org. For prices or further information, please inquire at srp@jax.org or nmf-mice@jax.org.
Contact e-Mail Address
 nmf-mice@jax.org