Family Identifier |
NMF253 |
Synonym(s) |
C57BL/6J-nmf253/JMon |
Synopsis |
Mutants are polydipsic. |
Gene Symbol |
nmf253 (View MGI Record) |
Gene |
neuroscience mutagenesis facility253 |
Allele Symbol |
nmf253 (View MGI Record) |
Allele |
neuroscience mutagenesis facility253 |
Abnormal Assay(s) |
Assay: Two-bottle choice taste test
Domain(s): Gustation/Olfaction
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Phenotype Description |
Two-month old homozygous mutants drink ~ 40% more water than wild-type mice, i.e. 7.3 ml/mouse/day for presumed n/n N3F3 compared to 5.4 ml/mouse/day for unaffected G3. N3F1 offspring was obtained from mating one phenodeviant G3 female (presumed n/n) with a wild-type C57BL/6J male (+/+). N3F1 males and females (presumed n/+) were intercrossed to produce N3F2 offspring. Water intake in the resulting 41 N3F2 mice was bimodally distributed: water intake in one group of 26 mice ranged from 4.4 to 6.4 ml/mouse/day (average 5.7 ml; presumed +/+ and n/+); in another group of 15 mice, water intake ranged from 6.8 to 8.5 ml/mouse/day (average 7.4 ml; presumed n/n). The N3F2 polydipsic mice (presumed n/n) were intercrossed to obtain N3F3 offspring. Average water intake in the eight N3F3 mice was 7.3 ml/mouse/day, consistent with an expected n/n genotype.
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Pathology Report |
No patholoy examination was performed. |
HERITABILITY AND MAPPING INFORMATION
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Background Strain |
C57BL/6J |
Heritability Mode |
Recessive |
Heritability Status |
Proven; eight of 51 G3 mice of this family were polydipsic. In N3F2, the ratio of the polydipsic and normal-drinking mice (15:26) was not significantly different from the expected ratio of 1(n/n):3 (n/+ and +/+); p = 0.09, chi-square test. N3F2 polydipsic mice (presumed n/n) were intercrossed to obtain N3F3 offspring. The N3F3 water intake was consistent with an expected n/n genotype.
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STATUS INFORMATION
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Mutant Status |
Cryopreserved Embryos
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Comments |
Please note: This mutation has not been chromosomally mapped - an important part of determining whether it is allelic with a previously characterized mutation. |
Contact e-Mail Address |
nmf-mice@jax.org |