STRAIN INFORMATION
Family Identifier
 NMF148
Synonym(s)
 C57BL/6J-nmf148/J; JR#4765
Synopsis
 Small, body tremor, severely impaired gait.
Gene Symbol
 nmf148 (View MGI Record)
Gene
 neuroscience mutagenesis facility148
Allele Symbol
 nmf148 (View MGI Record)
Allele
 neuroscience mutagenesis facility148
Abnormal Assay(s)
 Assay: Overt movement/posture

   Domain(s): Visible Mutants ; Movement/Neuromuscular Function

Assay: Body weight

   Domain(s): Growth/Development
Phenotype Description
 The mutants are small and show an intense body tremor with unsteady, 'seal-like' gait; their hind limbs appear weak, i.e. appear to carry the body weight with difficulty only, and sometimes one or the other hind limb lags behind. The phenotype can be observed at 3.5 weeks of age (+/-0.63; n=118). Mice of either gender are affected. A colony needs to be maintained through ovarian transplants and heterozygote matings.
Pathology Report
 Standard pathology work-up on two mutants (27 or 28 days of age) revealed a hypoplastic spleen with atrophy of both lymphoid and hemaopoietic areas, and atrophy of the thymus in both mice. Hypoplastic bone marrow was noted in one, and fatty changes in the liver were noted in the other animal.
HERITABILITY AND MAPPING INFORMATION
Background Strain
 C57BL/6J
Heritability Mode
 Recessive
Heritability Status
 Proven; 2 heterozygote matings produced 4 affected mice in a total of 10 progeny.
Chromosome
 11
Molecular Interval
 D11Mit145 - 5.8 cM - nmf148 - 8.4 cM - D11Mit181; linkage D11Mit14 5- LOD = 9.1; D11Mit18 1- LOD = 7.8
Map
STATUS INFORMATION
Mutant Status
 Cryopreserved Embryos
Comments
 Please note: The majority of frozen NMF embryos is obtained through matings between homozygous (or heterozygous) mutant males and wild-type females; if recovery of mutants is requested, the mice will be shipped as soon as possible following wean without further phenotype or genotype testing. NMF embryos are supplied subject to the General Terms and Conditions of Sale posted at www.jaxmice.jax.org. For prices or further information, please inquire at srp@jax.org or nmf-mice@jax.org. (3) NMF148 or DNA have been requested by and distributed to 2 investigators.
Contact e-Mail Address
 nmf-mice@jax.org