STRAIN INFORMATION
Family Identifier
 NMF65
Synonym(s)
 C57BL/6J-nmf65/J; JR#4470
Synopsis
 Low grip strength and hind limb weakness.
Gene Symbol
 nmf65 (View MGI Record)
Gene
 neuroscience mutagenesis facility65
Allele Symbol
 nmf65 (View MGI Record)
Allele
 neuroscience mutagenesis facility65
Abnormal Assay(s)
 Assay: Grip strength

   Domain(s): Movement/Neuromuscular Function

Assay: Overt movement/posture

   Domain(s): Visible Mutants

Assay: Body weight

   Domain(s): Growth/Development
Phenotype Description
 Mutants are small and show reduced grip strength (< 2 S.D. B6 mean) at 12 weeks of age. At 16 weeks of age they show an unsteady gait and labored breathing. By 17 weeks of age the mice appear extremely wasted.
Pathology Report
 Standard pathology work-up on three mutants revealed mild myopathy in all muscles characterized by scattered small muscle fibers often with central nuclei. Whole muscle mounts of hind limb stained with flurorescent-labeled bungarotoxin, SV2 and SMI 31 revealed no abnormalities of neuromuscular junctions. GFAP immunohistochemistry of brain and spinal cord showed gliosis of white matter in medulla and spinal cord. In dorsal columns of spinal cord the sensory tracts were quite severely gliotic, but the corticospinal tract was normal. A few dystrophic axons were observed in low lumbar spinal cord.
HERITABILITY AND MAPPING INFORMATION
Background Strain
 C57BL/6J
Heritability Mode
 Recessive
Heritability Status
 Proven; 3 heterozygous matings produced 3 affected mice in a total of 17 progeny.
Chromosome
 11
Molecular Interval
 D11Mit368 - 6.0 cM - nmf65 - 7.8 cM - D11Mit41
Map
STATUS INFORMATION
Mutant Status
 Cryopreserved Embryos
Comments
 Please note: The majority of frozen NMF embryos is obtained through matings between homozygous (or heterozygous) mutant males and wild-type females; if recovery of mutants is requested, the mice will be shipped as soon as possible following wean without further phenotype or genotype testing. NMF embryos are supplied subject to the General Terms and Conditions of Sale posted at www.jaxmice.jax.org. For prices or further information, please inquire at srp@jax.org or nmf-mice@jax.org. (3) NMF65 or DNA have been requested by and distributed to 2 investigators.
Contact e-Mail Address
 nmf-mice@jax.org