STRAIN INFORMATION
Family Identifier
 NMF62
Synonym(s)
 C57BL/6J-nmf62/J; JR#4442
Synopsis
 Low grip strength and abnormal gait.
Gene Symbol
 nmf62 (View MGI Record)
Gene
 neuroscience mutagenesis facility62
Allele Symbol
 nmf62 (View MGI Record)
Allele
 neuroscience mutagenesis facility62
Abnormal Assay(s)
 Assay: Grip strength

   Domain(s): Movement/Neuromuscular Function

Assay: Overt movement/posture

   Domain(s): Visible Mutants ; Movement/Neuromuscular Function
Phenotype Description
 The mutants were originally detected by reduced (<= 2 s.d. B6 mean) forelimb grip strength at 12 weeks of age. Subsequent observations revealed subtle abnormalities in their gait that can be detected at approximately 4 weeks of age (average on-set 4.3+/-0.75 weeks; n=33). In the interest of expediting availability, only the early visible phenotype was pursued.
Pathology Report
 Standard pathology work-up on two mutants (31 or 282 days of age)did not reveal any abnormalities. Whole muscle mounts of the hind limb stained with fluorescent-labeled bungarotoxin, SV2, and SMI 31 revealed no abnormalities of neuromuscular junctions. Additional pathology on brain, spinal cord, muscle and eyes performed on three other mutants (24 or 114 days of age) revealed retinal dysplasia in the older mutant. The eyes of all other mutants appeared normal.
HERITABILITY AND MAPPING INFORMATION
Background Strain
 C57BL/6J
Heritability Mode
 Recessive
Heritability Status
 Proven; 2 heterozygote matings produced 4 affected mice in a total of 19 progeny; 1 heterozygote x homozygote mating produced 5 affected in a total of 14 progeny.
Chromosome
 2
Molecular Interval
 D2Mit447 - 6.8 cM - nmf62 - 7.9 cM - D2Mit407
Map
STATUS INFORMATION
Mutant Status
 Cryopreserved Embryos
Comments
 Please note: The majority of frozen NMF embryos is obtained through matings between homozygous (or heterozygous) mutant males and wild-type females; if recovery of mutants is requested, the mice will be shipped as soon as possible following wean without further phenotype or genotype testing. NMF embryos are supplied subject to the General Terms and Conditions of Sale posted at www.jaxmice.jax.org. For prices or further information, please inquire at srp@jax.org or nmf-mice@jax.org. (7) NMF62 have been requested by and distributed to 2 investigators.
Contact e-Mail Address
 nmf-mice@jax.org